Extractive Cloud Point Spectrophotometric Determination of Mesalazine Using Brilliant Green Dye

A simple and accurate cloud point-spectrophotometric method for determination of Mesalazine (MEZ) in pure form pharmaceutical formulations is proposed. The procedure based on the reaction with brilliant green dye (BG) forming a pale-yellow ion-pair complex after extracting Triton X-114 measured at 361 nm. experimental conditions phase separation were optimized. Beer’s law was obeyed range 0.3-3.5 µg mL-1 molar absorptivity 3.914×104 L.mol-1.cm-1, average recovery % 100.82 relative standard deviation (RSD) 0.87. applied successfully MEZ its